Uses of Urease Test This test is used to differentiate organisms based on their ability to hydrolyze urea with the enzyme urease. It is also useful to identify Cryptococcus spp., Brucella, Helicobacter pylori, and many other bacteria that produce the urease enzyme.Click to see full answer. Subsequently, one may also ask, what is the purpose of the urease test?Urease Test. Urease broth is a differential medium that tests the ability of an organism to produce an exoenzyme, called urease, that hydrolyzes urea to ammonia and carbon dioxide. The broth contains two pH buffers, urea, a very small amount of nutrients for the bacteria, and the pH indicator phenol red.Beside above, how is urease test performed? The rapid urease test involves incubating a gastric biopsy in a urea broth that contains the pH indicator phenol red. If gastric helicobacters are present, helicobacter urease breaks down the urea; with the release of ammonia, a rise in pH and a color change occur. Also know, what does urease test mean? Rapid urease test, also known as the CLO test (Campylobacter-like organism test), is a rapid diagnostic test for diagnosis of Helicobacter pylori. The basis of the test is the ability of H. pylori to secrete the urease enzyme, which catalyzes the conversion of urea to ammonia and carbon dioxide.What bacteria are urease positive? Urease-positive pathogens include: Proteus mirabilis and Proteus vulgaris. Ureaplasma urealyticum, a relative of Mycoplasma spp. Nocardia. Corynebacterium urealyticum. Cryptococcus spp., an opportunistic fungus. Helicobacter pylori.
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